These findings would make it seem logical that the best course of action would be to develop inhibitors

Endogenous concentration of castasterone and inhibits BR-induced tracheary element differentiation. These reports suggested that Ucz may also affects BR biosynthesis and later screens of structurally similar azoles led to the development of brassinazole, the first specific BR biosynthetic inhibitor. Brzs mode of Eleutheroside E action is to bind and block DWF4/CYP90B1. DWF4 is a P450 that mediates multiple rate-limiting C22 alphahydroxylations in the biosynthesis of BRs.DWF4 expression is a target of regulation by both endogenous signals like auxin and exogenous cues like temperature. Brz and its even more specific derivative Brz2001 became invaluable tools for BR research. Not only did they help to reveal the role of BRs in various plant species, they were also essential for the isolation and characterization of genes that function in BR signaling. However, low accessibility and high costs associated with Brz/ Brz2001 have limited their use in agricultural crops that often require large-scale applications. In this context, it would be beneficial to the research community to have access to potent, specific, and more cost efficient azole BR inhibitors in plants. The triazole compound propiconazole as a potent inhibitor of BR biosynthesis was first reported after examining its inhibitory effect on hypocotyl elongation of cress plants. This inhibitory effect of Pcz was reversed by co-application with brassinolide. Based on the Pcz structure additional BR inhibitors were identified. On the other hand, Pcz has been commercially used as fungistat against a broad range of phytopathogenic fungi. Its fungistatic mode of action is the same as that of Ucz and Pac; blocking of lanosterol 14R-demethylase. Pcz has also been studied extensively for its toxicity on plants, animals, humans, and the environment. Here we present a molecular genetic analysis of Pczs effects on Arabidopsis and maize seedlings. Phytohormone biosynthesis inhibitors allow the species-independent study of hormonal function during plant development. Inhibitor studies can also support the isolation and characterization of hormone deficient mutants without prior knowledge of the mutant Sodium laureth sulfate supplier phenotype. Pcz has previously been reported to impair the hypocotyl growth of cress seedlings and that this inhibition is reversible b