Epresentative of OsGRF4 promoter haplotypes A, B and C (see most important text) are shown.

Epresentative of OsGRF4 promoter haplotypes A, B and C (see most important text) are shown. e, OsGRF4 mRNA abundance in numerous rice varieties below the high N circumstances (1.25 mM NH4NO3), OsGRF4 promoter haplotypes as indicated. Abundance data is all relative for the abundance of rice Actin2 mRNA. Information shown as imply s.e.m. (n = 3). Diverse letters denote considerable variations (P 0.05, Duncan’s a number of range test). f, Comparisons of OsGRF4 mRNA abundance in selected rice varieties grown in among high (HN, 1.25 mM NH4NO3) and low (LN, 0.375 mM NH4NO3) N circumstances. Information shown as mean s.e.m. (n = 3). Abundance information is all relative to that in HN (set to one). P 0.05 as compared to HN by two-sided Student’s ttest. g, Relative abundances of rice OsmiR396 family members in NJ6 plants grown at distinct levels of N supply (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3), shown relative to abundance in plants grown in 1N conditions (set to one particular). Data shown as imply s.e.m. (n = three). Various letters denote substantial variations (P 0.05, Duncan’s numerous range test).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsNature. Author manuscript; offered in PMC 2019 February 15.Li et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure 2. Comparisons NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 isogenic line 5-Acetylsalicylic acid site traits reveals that OsGRF4 regulates expression of NH4+ metabolism genes.a, Malachite green Cancer Mature plant height. Data shown as imply s.e.m. (n = 16). Distinctive letters denote substantial variations (P 0.05, Duncan’s various range test). b, The amount of tillers per plant. c, The number of grains per panicle. Information shown as mean s.e.m. (n = 16). Unique letters denote significant differences (P 0.05, Duncan’s numerous variety test). d, Flag-leaf width. Data shown as imply s.e.m. (n = 16). Distinct letters denote considerable variations (P 0.05, Duncan’s many variety test). e, Culm (stem) width expressed as diameter from the uppermost internode. Information shown as imply s.e.m. (n = 16). Unique letters denote important differences (P 0.05, Duncan’s numerous variety test). f, Grain yield per plant. Information shown as imply s.e.m. (n = 220). Diverse letters denote important differences (P 0.05, Duncan’s various variety test). g, Relative root abundance of OsAMT1.2 mRNA in NILs, genotypes as indicated. Abundance shown relative to that in NJ6 plants (=1). Data shown as mean s.e.m. (n = three). Various letters denote substantial variations (P 0.05, Duncan’sNature. Author manuscript; out there in PMC 2019 February 15.Li et al.Pagemultiple range test). h, Root glutamine synthase (GS) activities. Data shown as imply s.e.m. (n = 3). Diverse letters denote substantial variations (P 0.05, Duncan’s several variety test). i, Relative shoot abundance of OsFd-GOGAT mRNA. Abundance shown relative to that in NJ6 plants (=1). Information shown as imply s.e.m. (n = 3). Unique letters denote considerable differences (P 0.05, Duncan’s many variety test). j, Shoot glutamine synthase (GS) activities. Information shown as imply s.e.m. (n = 3). Various letters denote important variations (P 0.05, Duncan’s numerous variety test). k-n, Flag-OsGRF4 mediated ChIP-PCR enrichment (relative to input) of GCGG-containing promoter fragments (marked with ) from OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters. Diagrams depict putative OsAMT1.two, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters.