Epresentative of OsGRF4 promoter haplotypes A, B and C (see primary text) are shown. e,

Epresentative of OsGRF4 promoter haplotypes A, B and C (see primary text) are shown. e, OsGRF4 mRNA abundance in numerous rice varieties under the high N conditions (1.25 mM NH4NO3), OsGRF4 promoter haplotypes as indicated. Abundance data is all relative to the abundance of rice Actin2 mRNA. Information shown as mean s.e.m. (n = 3). Distinct letters D-Ribonolactone supplier denote substantial differences (P 0.05, Duncan’s multiple variety test). f, Comparisons of OsGRF4 mRNA abundance in selected rice varieties grown in amongst higher (HN, 1.25 mM NH4NO3) and low (LN, 0.375 mM NH4NO3) N circumstances. Data shown as imply s.e.m. (n = 3). Abundance data is all relative to that in HN (set to 1). P 0.05 as in comparison with HN by two-sided Student’s ttest. g, Relative abundances of rice OsmiR396 family members in NJ6 plants grown at various levels of N TMS Biological Activity supply (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3), shown relative to abundance in plants grown in 1N conditions (set to a single). Information shown as mean s.e.m. (n = three). Distinctive letters denote considerable variations (P 0.05, Duncan’s several variety test).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsNature. Author manuscript; obtainable in PMC 2019 February 15.Li et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure 2. Comparisons NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 isogenic line traits reveals that OsGRF4 regulates expression of NH4+ metabolism genes.a, Mature plant height. Information shown as mean s.e.m. (n = 16). Distinctive letters denote considerable variations (P 0.05, Duncan’s various variety test). b, The number of tillers per plant. c, The number of grains per panicle. Data shown as imply s.e.m. (n = 16). Distinctive letters denote significant differences (P 0.05, Duncan’s multiple variety test). d, Flag-leaf width. Information shown as imply s.e.m. (n = 16). Unique letters denote significant differences (P 0.05, Duncan’s multiple variety test). e, Culm (stem) width expressed as diameter on the uppermost internode. Information shown as imply s.e.m. (n = 16). Diverse letters denote substantial differences (P 0.05, Duncan’s several variety test). f, Grain yield per plant. Data shown as mean s.e.m. (n = 220). Distinct letters denote considerable variations (P 0.05, Duncan’s several variety test). g, Relative root abundance of OsAMT1.2 mRNA in NILs, genotypes as indicated. Abundance shown relative to that in NJ6 plants (=1). Data shown as imply s.e.m. (n = three). Distinct letters denote important variations (P 0.05, Duncan’sNature. Author manuscript; offered in PMC 2019 February 15.Li et al.Pagemultiple variety test). h, Root glutamine synthase (GS) activities. Information shown as imply s.e.m. (n = three). Distinct letters denote significant variations (P 0.05, Duncan’s multiple range test). i, Relative shoot abundance of OsFd-GOGAT mRNA. Abundance shown relative to that in NJ6 plants (=1). Information shown as mean s.e.m. (n = three). Various letters denote substantial variations (P 0.05, Duncan’s a number of variety test). j, Shoot glutamine synthase (GS) activities. Data shown as imply s.e.m. (n = three). Various letters denote considerable differences (P 0.05, Duncan’s numerous range test). k-n, Flag-OsGRF4 mediated ChIP-PCR enrichment (relative to input) of GCGG-containing promoter fragments (marked with ) from OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters. Diagrams depict putative OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters.