Epresentative of OsGRF4 promoter haplotypes A, B and C (see primary text) are shown. e,

Epresentative of OsGRF4 promoter haplotypes A, B and C (see primary text) are shown. e, OsGRF4 mRNA abundance in several rice varieties beneath the high N situations (1.25 mM NH4NO3), OsGRF4 promoter haplotypes as indicated. Abundance Uridine 5′-monophosphate Formula Information is all relative to the abundance of rice Actin2 mRNA. Information shown as imply s.e.m. (n = three). Unique letters denote considerable variations (P 0.05, Duncan’s numerous range test). f, Comparisons of OsGRF4 mRNA abundance in selected rice varieties grown in between high (HN, 1.25 mM NH4NO3) and low (LN, 0.375 mM NH4NO3) N conditions. Information shown as imply s.e.m. (n = three). Abundance information is all relative to that in HN (set to one). P 0.05 as in comparison to HN by two-sided Student’s ttest. g, Relative abundances of rice OsmiR396 family members in NJ6 plants grown at distinctive levels of N provide (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3), shown relative to abundance in plants grown in 1N circumstances (set to one particular). Information shown as imply s.e.m. (n = three). Unique letters denote substantial variations (P 0.05, Duncan’s numerous range test).Europe PMC Funders Author Manuscripts Europe PMC Funders Author (S)-(-)-Limonene Description ManuscriptsNature. Author manuscript; readily available in PMC 2019 February 15.Li et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Information Figure 2. Comparisons NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 isogenic line traits reveals that OsGRF4 regulates expression of NH4+ metabolism genes.a, Mature plant height. Information shown as mean s.e.m. (n = 16). Unique letters denote significant differences (P 0.05, Duncan’s various range test). b, The amount of tillers per plant. c, The amount of grains per panicle. Information shown as imply s.e.m. (n = 16). Different letters denote considerable variations (P 0.05, Duncan’s multiple variety test). d, Flag-leaf width. Data shown as imply s.e.m. (n = 16). Unique letters denote important differences (P 0.05, Duncan’s numerous variety test). e, Culm (stem) width expressed as diameter on the uppermost internode. Data shown as mean s.e.m. (n = 16). Unique letters denote important differences (P 0.05, Duncan’s several range test). f, Grain yield per plant. Information shown as imply s.e.m. (n = 220). Unique letters denote considerable variations (P 0.05, Duncan’s a number of variety test). g, Relative root abundance of OsAMT1.two mRNA in NILs, genotypes as indicated. Abundance shown relative to that in NJ6 plants (=1). Data shown as imply s.e.m. (n = three). Distinctive letters denote considerable variations (P 0.05, Duncan’sNature. Author manuscript; offered in PMC 2019 February 15.Li et al.Pagemultiple range test). h, Root glutamine synthase (GS) activities. Information shown as mean s.e.m. (n = 3). Distinctive letters denote considerable differences (P 0.05, Duncan’s various variety test). i, Relative shoot abundance of OsFd-GOGAT mRNA. Abundance shown relative to that in NJ6 plants (=1). Information shown as mean s.e.m. (n = three). Unique letters denote considerable variations (P 0.05, Duncan’s multiple range test). j, Shoot glutamine synthase (GS) activities. Data shown as mean s.e.m. (n = 3). Different letters denote substantial variations (P 0.05, Duncan’s multiple variety test). k-n, Flag-OsGRF4 mediated ChIP-PCR enrichment (relative to input) of GCGG-containing promoter fragments (marked with ) from OsAMT1.two, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters. Diagrams depict putative OsAMT1.two, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters.