Representing individual proteins were divided in 4 distinctive groups: p-value 0.05 (black); p-value 0.05 (grey);

Representing individual proteins were divided in 4 distinctive groups: p-value 0.05 (black); p-value 0.05 (grey); p-value 0.05, 1.5-fold greater GSK3987 Autophagy abundance in colon (orange); p-value 0.05, 1.5-fold larger abundance in spleen (blue). For both sample groups two proteins, getting typical higher abundant in colon or spleen tissue, respectively are highlighted and named (see Supplementary Table S4).As expected, there are proteins with characteristic abundance in colon and spleen tissue, respectively. These proteins are visible within the volcano plot in Figure 4 as colored dots. Proteins with comparable abundances cover about 65 on the colon and spleen tissues. These proteins belong for the basic inventory which each cell demands, independently from its specialization. Within the study of Lee et al. (2016) a list of 20 newly identified housekeeping proteins was published showing a uniform Protein abundance level (CV 20 , 1.5-fold transform) over 27 diverse tissue types [29]. In Figure 5 a relative protein abundance plot is shown for 4 examples of those housekeeping proteins.Int. J. Mol. Sci. 2021, 22,7 ofFigure five. Relative protein abundance plots for (a) Poly(rC)-binding protein 1 (Pcbp1), (b) 26S protease regulatory subunit 10B (Psmc6), (c) 26S proteasome non-ATPase regulatory subunit 7 (Psmd7) and (d) Serine/arginine-rich splicing factor 1 (Srsf1) in murine colon and spleen samples. The data in (a) are highlighted in orange for colon tissue and in blue for spleen tissue.Figure 5 shows very similar relative protein abundances for the colon and spleen 1-Aminocyclopropane-1-carboxylic acid-d4 Formula samples for the 4 selected housekeeping proteins. Based on this, we can confirm that the specific variations in between the colon and spleen samples are indeed due to drastically distinct protein abundances in the tissues and not on account of, as an example, various suitability of both organs for tissue sampling by way of NIRL. For further validation, two example proteins of every sample group with characteristic abundance within the respective tissues have been highlighted and named in Figure 4. For the colon tissue samples, the proteins hemoglobin subunit alpha (Hba) and coactosin like F-actin binding protein 1 (Cotl1) are drastically larger in abundance. The proteins Keratin, sort I cytoskeletal 19 (Krt19) and sodium/potassiumtransporting ATPase subunit alpha-1 (Atp1a1) show a 1.5-fold greater abundance in spleen tissue when compared with the colon tissue samples. For these proteins, the relative protein abundances are compared against the corresponding protein abundance data in the Human Protein Atlas version 20.1 (http://www.proteinatlas.org, accessed on 15 July 2020) [30] within the following Figures six and 7.Int. J. Mol. Sci. 2021, 22,8 ofFigure 6. Relative protein abundance plots for (a) hemoglobin subunit alpha (Hba) and (c) coactosin like F-actin binding protein 1 (Cotl1) in murine colon and spleen samples. The corresponding protein abundance data of the Human Protein Atlas (HPA) [30] is shown in (b) for Hba and (d) for Cotl1. A total of 44 different tissue kinds were examined for the protein abundance information in the HPA. The information in (a,c) is highlighted in orange for colon tissue and in blue for spleen tissue. Photos (b,d) are readily available from v20.1.proteinatlas.org.The relative abundances of each proteins Hba and Cotl1 (see Supplementary Table S4) were plotted for the two various tissues, colon and spleen in Figure six. In addition, the protein abundance information from the Human Protein Atlas for Hba and Cotl1 are shown. Bo.