Seeds are visible, sheath open Seed formation V V Green/brown spathe, dark brown seeds are

Seeds are visible, sheath open Seed formation V V Green/brown spathe, dark brown seeds are visible, sheath openFigure 2. Figure two. Morphology from the 5 sexual reproductive stages (I-V)(I ) determined to the repro- reproducMorphology from the five sexual reproductive stages determined to study study the ductive phenology in the Z. noltei meadows in the Ria de Aveiro lagoon. tive phenology of the Z. noltei meadows at the Ria de Aveiro lagoon.The total number of spathes collected in every reproductive stage through the study period was calculated to describe the basic reproductive phenology of Z. noltei within the To test the germination capability of your Z. noltei meadows, all collected sexual Ria de Aveiro lagoon. To test differences among meadows within the reproductive phenology, spathes, no matter their stage in each reproductive stage have been separated per meadowdate. YTX-465 MedChemExpress sampling the percentage of spathes of sexual reproduction, was estimated by meadow and and2.three. Germination Abilitydate and cultured in aquaria (ten cm of diameter, filled with 600 mL LY294002 medchemexpress artificial seawater) until two.three. Germination Ability mature seeds had been obtained to apply for germination tests. To test the germination potential of was noltei meadows, 72 h for the mean seawater The salinity in every single aquariumthe Z.adjusted every all collected sexual spathes, salinity irrespective of theirZ. noltei meadow in the corresponding sampling date (Table S1). Aquaria obtained in every single stage of sexual reproduction, had been separated per meadow and sampling date and cultured in aquaria (10 cm of diameter, filled with 600 mL artificial seawere aerated (Air 550 R Plus, Sera GmbH, Heinsberg, Germany; O2 flux 5 mg/L) and water) till mature seeds were obtained to apply for germination tests. maintained at room temperature (from 18.35 0.20 C to 23.66 0.12 C) beneath natural The salinity in every aquarium was adjusted every 72 h to the imply seawater salinity photoperiod (from 14:ten to 12:12 the corresponding sampling date2019 toS1). Aquaria light/dark hours, from July (Table March 2020). Each obtained in every Z. noltei meadow in week, aerated (Air 550 with challenging, dark brown seed coat, were O2 flux five counted and stored were mature seeds, R Plus, Sera GmbH, Heinsberg, Germany; collected, mg/L) and at salinitiesat area 33 psu in 50 mL tubes illed to 23.66 0.12seawater to avoid natural maintained above temperature (from 18.35 0.20 with artificial ) under organic seed germination ahead of the test. The seeds were kept stored in the tubes till March 2020, when the germination tests were carried out, coinciding with all the starting of spring as well as the organic germination period of the species [29]. Spathes that suffered senescence were removed in the aquaria to prevent their decomposition. To stop overgrowth of epiphytes, artificial seawater was renewed, and the aquaria and aeration technique had been cleaned when a week. Seed production was expressed as the variety of seeds per spathe. Germination tests have been carried out by exposing mature seeds to a low salinity shock to induce germination [12]. Seeds had been introduced into aerated tubes (50 mL) filled withPlants 2021, 10,5 of20 mL of 1 psu artificial seawater and 20 mL of agar (5 at 1 psu) as neutral substrate and placed inside an environmental chamber with controlled temperature and photoperiod (25.00 1.026 C, 12:12 light/dark hours, respectively). Then, the number of germinated seeds (with broken seed coat and visible cotyledon) was recorded every week for 4 weeks to ca.