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Itions. J Am Chem Soc 131(2):42627. 28. Partch CL, Clarkson MW, Ozg S
Itions. J Am Chem Soc 131(2):42627. 28. Partch CL, Clarkson MW, Ozg S, Lee AL, Sancar A (2005) Part of structural plasticity in signal transduction by the cryptochrome blue-light photoreceptor. Biochemistry 44(ten):3795805. 29. Antony J, Medvedev DM, Stuchebrukhov AA (2000) Theoretical study of electron transfer in between the photolyase catalytic cofactor FADH- and DNA thymine dimer. J Am Chem Soc 122(6):1057065. 30. Web page CC, Moser CC, Chen XX, Dutton PL (1999) All-natural engineering principles of electron tunnelling in biological oxidation-reduction. Nature 402(6757):472. 31. Maul MJ, et al. (2008) Crystal structure and mechanism of a DNA (6-4) photolyase. Angew Chem Int Ed Engl 47(52):100760080. 32. Li J, Uchida T, Todo T, Kitagawa T (2006) Similarities and differences amongst cyclobutane pyrimidine dimer photolyase and (6-4) photolyase as revealed by resonance Raman spectroscopy: Electron transfer from the FAD cofactor to ultravioletdamaged DNA. J Biol Chem 281(35):255515559.Liu et al.PNAS | August 6, 2013 | vol. 110 | no. 32 |CHEMISTRYBIOPHYSICS AND COMPUTATIONAL BIOLOGY
Lau et al. BMC Complementary and Alternative Medicine 2013, 13:313 http:biomedcentral1472-688213RESEARCH ARTICLEOpen AccessNovel angiotensin I-converting enzyme inhibitory peptides derived from an edible mushroom, Pleurotus cystidiosus O.K. Miller identified by LC-MSMSChing Ching Lau1, Noorlidah Abdullah1 and Adawiyah Suriza Shuib1,AbstractBackground: Angiotensin I-converting enzyme (ACE) inhibitors have been reported to decrease mortality in patients with hypertension. In comparison with chemosynthetic drugs, ACE inhibitors derived from organic sources which include meals proteins are believed to become safer for consumption and to possess fewer adverse effects. Some edible mushrooms have been reported to significantly decrease blood pressure soon after oral administration. Also, mushrooms are identified to be rich in protein content. This makes them a potential source of ACE inhibitory peptides. Hence, the objective from the current study was to isolate and characterise ACE inhibitory peptides from an edible mushroom, Pleurotus cystidiosus. Techniques: ACE inhibitory proteins have been isolated from P. cystidiosus determined by the bioassay guided purification actions, i.e. ammonium sulphate precipitation, reverse phase high efficiency liquid chromatography and size exclusion chromatography. Active fraction was then analysed by LC-MSMS and potential ACE inhibitory peptides identified had been chemically synthesized. Effect of in vitro gastrointestinal digestions around the ACE inhibitory HD2 Compound activity of the peptides and their inhibition patterns had been evaluated. Results: Two potential ACE inhibitory peptides, AHEPVK and GPSMR were identified from P. cystidiosus with molecular masses of 679.53 and 546.36 Da, respectively. Both peptides exhibited potentially high ACE inhibitory activity with IC50 values of 62.eight and 277.5 M, respectively. SEC chromatograms and BIOPEP analysis of those peptides revealed that the peptide sequence on the hexapeptide, AHEPVK, was steady throughout gastrointestinal digestion. The pentapeptide, GPSMR, was hydrolysed right after digestion and it was predicted to release a dipeptide ACE inhibitor, GP, from its precursor. The Lineweaver-Burk plot of AHEPVK showed that this potent and stable ACE inhibitor features a competitive inhibitory impact Caspase 2 web against ACE. Conclusion: The present study indicated that the peptides from P. cystidiosus could be potential ACE inhibitors. Despite the fact that these peptides had reduced ACE inhibitor.

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