Stiansen EN, Lund JS, Rortveit T, Rustan AC: Impact of dietary n-3 and n-6 fatty

Stiansen EN, Lund JS, Rortveit T, Rustan AC: Impact of dietary n-3 and n-6 fatty acids on fatty acid desaturation in rat liver. Biochim Biophys Acta 1991, 1082:57?two. 46. Igarashi M, Ma K, Chang L, Bell JM, Rapoport SI: Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain. J Lipid Res 2007, 48:2463?470. 47. Tu WC, Cook-Johnson RJ, James MJ, Muhlhausler BS, Gibson RA: Omega-3 extended chain fatty acid synthesis is regulated far more by substrate levels than gene expression. Prostaglandins Leukot Essent Fatty Acids 2010, 83:61?eight.doi:10.1186/1476-511X-12-147 Cite this short article as: Casey et al.: Impact of stearidonic acid-enriched soybean oil on fatty acid profile and metabolic parameters in lean and obese Zucker rats. Lipids in Well being and Disease 2013 12:147.
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. three, pp. 1319 ?331, January 16, 2015 ?2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.Involvement in the Na /Ca2 exchanger isoform 1 (NCX1) in Neuronal Development Aspect (NGF)-induced Neuronal Differentiation by means of Ca2 -dependent Akt PhosphorylationReceived for publication, July 7, 2014, and in revised type, November 21, 2014 Published, JBC Papers in Press, November 21, 2014, DOI 10.1074/jbc.M114.Agnese Secondo1, Alba Esposito1, Rossana Sirabella?, Francesca TrkC Activator Compound Boscia, Anna Pannaccione, Pasquale Molinaro, Maria Cantile, Roselia Ciccone, Maria Jos?Sisalli, Antonella Scorziello, Gianfranco Di Renzo, and Lucio Annunziato? In the Division of Neuroscience and Reproductive and Odontostomatological Sciences, College of Medicine, “Federico II” University of Naples, Through Sergio Pansini five, Naples 80131, Italy plus the �Fondazione Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) SDN, Naples 80143, ItalyBackground: NCX1 regulates intracellular Ca2 and Na homeostasis in neurons. Outcomes: The overexpression of NCX1 induced neuronal differentiation by means of Akt as well as NGF exposure. NCX1 knockdown prevented NGF-induced neurite outgrowth. Conclusion: NCX1 participates in neuronal differentiation by ionic regulation and Akt phosphorylation. Significance: Studying how NCX1 participates in neurite outgrowth will enhance the information of neuronal differentiation. NGF induces neuronal differentiation by modulating [Ca2 ]i. Even so, the part from the three isoforms in the major Ca2 -extruding program, the Na /Ca2 exchanger (NCX), in NGF-induced differentiation remains unexplored. We investigated whether NCX1, NCX2, and NCX3 isoforms could play a relevant function in neuronal differentiation by way of the modulation of [Ca2 ]i and the Akt pathway. NGF brought on progressive neurite elongation; a substantial improve with the well-known marker of development cones, GAP-43; and an enhancement of endoplasmic reticulum (ER) Ca2 content material and of Akt phosphorylation through an early activation of ERK1/2. Interestingly, for the duration of NGF-induced differentiation, the NCX1 protein level improved, NCX3 decreased, and NCX2 remained unaffected. In the exact same time, NCX total activity improved. In PPARβ/δ Antagonist Species addition, NCX1 colocalized and coimmunoprecipitated with GAP-43, and NCX1 silencing prevented NGF-induced effects on GAP-43 expression, Akt phosphorylation, and neurite outgrowth. Alternatively, the overexpression of its neuronal splicing isoform, NCX1.four, even in the absence of NGF, induced a rise in Akt phosphorylation and GAP-43 protein expression. Interestingly, tetrodotoxin-sensitive Na curren.