Gy Division, Instituto Nacional de Ciencias M icas y Nutrici Salvador

Gy Division, Instituto Nacional de Ciencias M icas y Nutrici Salvador Zubir , Mexico City 14080, Mexico Centro de Investigaciones Biom icas de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada No. 800, Col. Independencia, Guadalajara 44340, Mexico University Center of Biomedical Research, CONACyT-Universidad de Colima, Av. 25 de Julio 965, Col. Villas San Sebastian, Colima 28045, Mexico Correspondence: [email protected]; Tel./Fax: +52-312-316-1000 (ext. 70557 or 47452) These authors contributed equally to this perform.Citation: L ez, M.; Quintero-Mac s, L.; Huerta, M.; Rodr uez-Hern dez, A.; Melnikov, V.; C denas, Y.; Bricio-Barrios, J.A.; S chez-Pastor, E.; GamboaDom guez, A.; Leal, C.; et al. Capsaicin Decreases Kidney Iron Deposits and Increases Hepcidin Levels in Diabetic Rats with Iron Overload: A Preliminary Study. Molecules 2022, 27, 7764. doi.org/10.3390/molecules27227764 Academic Editor: Alessia Ligresti Received: 30 August 2022 Accepted: 9 November 2022 Published: 11 November 2022 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Abstract: Iron overload (IOL) increases the threat of diabetes mellitus (DM). Capsaicin (CAP), an agonist of transient receptor prospective vanilloid-1 (TRPV1), reduces the effects of IOL. We evaluated the effects of chronic CAP administration on hepcidin expression, kidney iron deposits, and urinary biomarkers in a male Wistar rat model with IOL and DM (DM-IOL). IOL was induced with oral administration of iron for 12 weeks and DM was induced with streptozotocin. 4 groups were studied: Healthy, DM, DM-IOL, and DM-IOL + CAP (1 mg g-1 ay-1 for 12 weeks). Iron deposits were visualized with Perls tissue staining and also a colorimetric assay. Serum hepcidin levels were measured with an enzymelinked immunosorbent assay. Kidney biomarkers were assayed in 24 h urine samples. In the DM-IOL + CAP group, the total area of iron deposits as well as the total iron content in kidneys were smaller than those observed in both untreated DM groups. CAP administration substantially elevated hepcidin levels in the DM-IOL group. Urinary levels of albumin, cystatin C, and beta-2-microglobulin were related in all 3 experimental groups. In conclusion, we showed that in a DM-IOL animal model, CAP lowered renal iron deposits and increased the amount of circulating hepcidin. Keywords: iron overload; capsaicin; diabetes mellitus; kidney; biomarkers1. Introduction Iron overload (IOL) is prevalent in kind two diabetes (DM) [1], but the underlying mechanisms haven’t been fully elucidated. The partnership among IOL and DM seems to become bidirectional, depending on reports which have shown that complications secondary to DM seem to be related to IOL. Each iron deficiency and IOL have already been linked with impaired glucose tolerance in DM [2]; thus, it really is not uncommon for DM and IOL to coexist within the very same individual.TMPRSS2 Protein custom synthesis Iron plays a crucial physiological role in a variety of metabolic pathways.Artemin Protein Source IOL disrupts iron homeostasis, which induces defective signaling.PMID:23935843 Hence, IOL causes alterations in metabolic activity via multiple mechanisms that contribute to DM, including gluconeogenesis, lipolysis, and processes that ascertain fuel availability. These alterations affect the pancreas, liver, adipocytes, and muscle, exactly where they result in decreased insulin secretion, insulin resistance, and enhanced hepatic gluconeogenesis. The precise mechanisms thatCopyright: 2022 by the authors. L.